Checking and preserving the quantitative intensity content of your images

As you learned in the previous session, images are basically a matrix of gray values. In this session you will practice different ImageJ tools to examine the gray values in your images. You will learn how to use these tools to identify problems and properties associated with fluorescence microscopy images, such as image offset, dynamic range and saturation. Next we will discuss the dangers when converting an image, e.g. from 16 to 8 bit and how to do this best, if you really need to. Finally there will be a practical session where you save an image in different file-formats and learn the consequences of this operation.