NEUBIAS TS2
NEUBIAS TS2
Assembling data for publication using FigureJ
Assembling data for publication using FigureJ
ImageJ, GUI Training for Beginners: learn how to use FigureJ
Building a workflow with CellProfiler
Building a workflow with CellProfiler
CellProfiler, Counting PLA dots, track swelling organoids
Checking and preserving the quantitative intensity content of your images
Checking and preserving the quantitative intensity content of your images
As you learned in the previous session, images are basically a matrix of gray values. In this session you will practice different ImageJ tools to examine the gray values in your images. You will learn how to use these tools to identify problems and properties associated with fluorescence microscopy images, such as image offset, dynamic range and saturation. Next we will discuss the dangers when converting an image, e.g. from 16 to 8 bit and how to do this best, if you really need to. Finally there will be a practical session where you save an image in different file-formats and learn the consequences of this operation.
From pixels to microns
From pixels to microns
ImageJ, GUI Training for Beginners: ROIs, image calibrations and simple measurements
From workflows to simple batch macros: Measure Phalloidin in the nucleus area.
From workflows to simple batch macros: Measure Phalloidin in the nucleus area.
ImageJ, for those with GUI knowledge but without scripting knowledge
From workflows to simple batch macros: Quantify intensity at the nuclear envelope.
From workflows to simple batch macros: Quantify intensity at the nuclear envelope.
ImageJ, for those with GUI knowledge but without scripting knowledge.
Introduction to ImageJ basic operations
Introduction to ImageJ basic operations
After a short historical introduction about ImageJ and its branches, participants will learn basics about the nature of an image: what do we call an image ? How is image data stored ? What is a file format ? Basic usage of ImageJ will then be addressed: how to open an image ? How to install add-ons ? Useful tips and tricks will also be taught.
Recording simple macros for batch processing
Recording simple macros for batch processing
ImageJ, for those with GUI knowledge but without scripting knowledge, workflow: MIP, change LUT, merge channels
Working with color images and images in color
Working with color images and images in color
ImageJ, GUI Training for Beginners: LUT, RGB, Composite
Working with objects: measurements in 2D and 3D
Working with objects: measurements in 2D and 3D
ImageJ, GUI training for beginners, Count and Measure 2D nuclei,Count number of foci on 3D image , compare 2D, 2.5D, 3D results (simple workflows)
Working with pixels: filters, morphomaths and binary operations
Working with pixels: filters, morphomaths and binary operations
Working with pixels: filters, morphomaths and binary operations: ImageJ, GUI training for beginners.
Working with stacks: 3D image visualisation
Working with stacks: 3D image visualisation
ImageJ, GUI Training for Beginners: Projection, Volume and surface rendering