large data

LOBSTER (Little Objects Segmentation and Tracking Environment), an environment designed to help scientists design and customize image analysis workflows to accurately characterize biological objects from a broad range of fluorescence microscopy images, including large images, i.e. terabytes of data, exceeding workstation main memory.

• 75 workflows available 
• no programming, with GUI
• matlab based 

HDF5 is a data format for storing extremely large and complex data collections. This Fiji/ImageJ HDF5 plugin saves and loads 2D - 5D datasets with flexible options.

In Fiji, the plugin is downloadable via update site "HDF5".

This workflow classifies objects based on object-level features (e.g. intensity based, morphology based, etc) and user annotations. It needs segmentation images besides the raw image data. Segmentation images can be obtained from ilastik pixel classification, or binary segmentation images from other tools. Within the object classification, one can prefilter objects through thresholds (on pixel probability image) or object sizes (on segmentation image). Outputs are predicted classification label images. Selected features can also be exported. Advanced users also have possibilities to add customized (object) features for classification in a simple plugin fashion through python scripts.