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Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2) has a different gene knocked down by RNAi.
The sample preparation is described in more detail by [Carpenter et al. (Genome Biology, 2006)(http://genomebiology.biomedcentral.com/articles/10.1186/gb-2006-7-10-r1…)