Kymograph generation under ImageJ:
one simple solution, plot a line (ROI line) on the first frame, where you want to generate the kymograph.
Image / Stacks / Reslice
It will generate a new image were Y dimension is the time, and X the position on the line you have drawn.
02/01/2018 - 20:07
02/23/2018 - 12:41