PAINT image reconstruction
STORM image reconstruction
SMLM image reconstruction
Single-molecule localisation microscopy image reconstruction
PALM image reconstruction

2D Gaussian fitting macro (Fiji/ImageJ) for multiple signals.


This script includes a rough feature detection and then fine 2D Gaussian algorithm to fit Gaussians within detected regions. This macro is unique because the ImageJ/Fiji curve fitting API only supports 1-D curve. I get around this by linearising the equation. This implementation is for isotropic (spherical) or anistropic (longer in x/y) diagonally covariant Gaussians but not fully covariant Gaussians (anisotropic and rotated). 

Detection of Molecules - DoM


A collection of components for super resolution image data:

  • Detect Molecules
  • Reconstruct Image
  • Results table
  • Drift correction
  • Chromatic correction

RapidSTORM usage tutorial


[as of 20180524, the website is temporary not functioning do to web defacement - please check again later]

This tutorial will exemplify basic rapidSTORM usage by showing how to convert an Andor SIF acquisition to a super-resoluted image with rapidSTORM.

Sample data is available: tubilin