Stitch Tiles, Flat Field Correction, Quantify ProtX intensity at Nuclei
ImageJ, for those with GUI knowledge but without scripting knowledge
A Component is an implementation of certain image processing / analysis algorithms.
Each component alone does not solve a Bioimage Analysis problem.
These problems can be addressed by combining such components into workflows.
ImageJ, for those with GUI knowledge but without scripting knowledge
In this session we will use a 3D multichannel reconstruction of zebrafish larva to explore the visualization capabilities of Matlab. We will start from extracting and inspecting single slices and will continue with combining multiple channels, finally generating a surface rendering for visual colocalization analysis.During the process we will review methods for manipulating multidimensional arrays, including resizing, reshaping and conditional selection.
Matlab is a commercial software for numerical computing and statistical analysis that can be used to process and analyze multidimensional images. Within this session you will get familiar with the Matlab environment and its programming language. Among the topics addressed: matrix manipulation and advanced indexing, access to files, data inspection and basic plotting. This session will provide you the foundations that you need for the following modules, where you will manipulate images, extract measurements, and statistically analyze and visualize results.
ImageJ, for those with GUI knowledge but without scripting knowledge, workflow: MIP, change LUT, merge channels
Lecture Slides