Linear transformation


CLIJ2 is a GPU-accelerated image processing library for ImageJ/FijiIcy, Matlab and Java. It comes with hundreds of operations for filteringbinarizinglabelingmeasuring in images, projectionstransformations and mathematical operations for images. While most of these are classical image processing operations, CLIJ2 also allows performing operations on matrices potentially representing neighborhood relationships between cells and pixels.

CLIJ2 was developed to process images from fluorescence microscopy data of developing cells, tissues, organoids and organisms.


FLIRT (FMRIB's Linear Image Registration Tool) is a fully automated robust and accurate tool for linear (affine) intra- and inter-modal brain image registration.

FLIRT comes with a main GUI as well as three supporting guis:

  • ApplyXFM - for applying saved transformations and changing FOVs
  • InvertXFM - for inverting saved transformations
  • ConcatXFM - for concatenating saved transformations

Using a text file containing 3D point coordinates as reference pairs, 3D image stack is transformed.