Spot detection

This workflow detects spots in a 2D image by filtering the image by Laplacian of Gaussian (user defined radius), thresholding (user defined threshold) and finding local intensity maxima in mask distance map (Dmap).

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Spot detection

Distance transform

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Working with objects: measurements in 2D and 3D

ImageJ, GUI training for beginners, Count and Measure 2D nuclei,Count number of foci on 3D image , compare 2D, 2.5D, 3D results (simple workflows)

Hands-on exercises for learning Imaris

Imaris, Visualization, spots segmentation, surfaces segmentation, colocalization, spots tracking, Batch, plotting graphs with Vantage.

Read more about Hands-on exercises for learning Imaris
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Spot detection

Spot Detection 3D Hessian (ImageJ)

Spot Detection 3D (Icy)

Spot Detection Dmap (ImageJ)

Working with objects: measurements in 2D and 3D

Hands-on exercises for learning Imaris