Stitch Tiles, Flat Field Correction, Quantify ProtX intensity at Nuclei
ImageJ, for those with GUI knowledge but without scripting knowledge
Tutorial
Introduction to ImageJ macros: Quantify the enrichment of NPC proteins at the nuclear envelope, relative to its cytoplasmic localisation
ImageJ, for those with GUI knowledge but without scripting knowledge
Working with objects: measurements in 2D and 3D
ImageJ, GUI training for beginners, Count and Measure 2D nuclei,Count number of foci on 3D image , compare 2D, 2.5D, 3D results (simple workflows)
Analysis of Microtubule Orientation: Tracking with ImageJ, Directionality Analysis with Matlab
We take an example image data of microtubule binding protein EB1, and will study how to automatically track those signals and how to analyze the tracking results. We use ImageJ for measuring the temporal changes in signal positions, and will feed the tracking results for analyzing their dynamics using Matlab in the following session.
Visualization of 3D images with Matlab
In this session we will use a 3D multichannel reconstruction of zebrafish larva to explore the visualization capabilities of Matlab. We will start from extracting and inspecting single slices and will continue with combining multiple channels, finally generating a surface rendering for visual colocalization analysis.During the process we will review methods for manipulating multidimensional arrays, including resizing, reshaping and conditional selection.