spots

Track non-dividing particles in 2D time-lapse image.

Particle tracking in 2D time-lapse based on linking closest regional intensity minima (user defined noise tolerance) detected from Laplacian of Gaussian filtered images (user defined radius). A maximum linking distance is set (user defined).

3D spot detection using the Determinant of Hessian (DoH) and the detection of 3D minima.