Component

Image correction software for chromatic shifts in fluorescence microscopy

Assess the performance of the lasers, the objective lenses and other key components required for optimum confocal operation.

The macro generates orthogonal projections from bead images along the lateral and axial dimensions which are displayed using a customized look-up-table to color code intensities. A Gaussian curve is fit to the intensity profile of a fluorescent bead image and full-with-at-half-maximum (FWHM) values are extracted, and listed next to theoretical values for comparison. 

Spimagine is a python package to interactively visualize and process time lapsed volumetric data as generated with modern light sheet microscopes (hence the Spim part). The package provides a generic 3D+t data viewer and makes use of GPU acceleration via OpenCL. If provides further an image processor interface for the GPU accelerated denoising and deconvolution methods of gputools.

It is only for display (no analysis). The only drawback: it does not handle multichannel time lapse 3D data (only one channel at a time).