particles

Track non-dividing particles in 2D time-lapse image.

Particle tracking in 2D time-lapse based on linking closest regional intensity minima (user defined noise tolerance) detected from Laplacian of Gaussian filtered images (user defined radius). A maximum linking distance is set (user defined).

3D spot detection using the Determinant of Hessian (DoH) and the detection of 3D minima.

This plugin ships automated methods for extracting trajectories of multiples objects in a sequence of 2D or 3D images. Up to version 2 it was known as the ‘Probabilistic particle tracker’ plugin.