Using ImageJ to Analyze the Size, Shape, and Directionality of Networks of Coupled Astrocytes

Type
Author
Steven Condamine
Dorly Verdier
Arlette Kolta
Requires
Execution Platform
Supported image dimension
Interaction Level
License/Openness
Description

The research goal of this paper was to provide unbiased counts of labeled astrocytes and to estimate the area they cover, further to develop tools for defining the orientation of coupling within astrocyte networks under different stimuli.

In order to count the astrocytes and estimate the area they cover the following steps were used in this software.

Pre-processing: z-project (using max intensity); split channels; subtract background; remove outliers.

Segmentation: adjust threshold and convert to a binary file; Watershed.

Cell counting: Analyze particles

Measure Astrocytic network area: select a ROI using the polygon tool; set measurements (area); ROI manager -> add the traced polygon; measure.

has biological terms
Additional keywords
Last modified
05/24/2023 - 17:28